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Effect of blood collection and processing on radioimmunoassay results for apolipoprotein B in plasma.

TitleEffect of blood collection and processing on radioimmunoassay results for apolipoprotein B in plasma.
Publication TypeJournal Article
Year of Publication1990
AuthorsBrown SA, Epps DF, Dunn JK, Sharrett AR, Patsch JR, Gotto AM, Patsch W
JournalClin Chem
Date Published1990 Sep
KeywordsAnalysis of Variance, Apolipoproteins B, Blood Specimen Collection, Humans, Radioimmunoassay, Specimen Handling

We studied the effects of different blood collection and processing procedures on quantification of apolipoprotein (apo) B by radioimmunoassay. High-density lipoprotein subfractions HDL3 and HDL2 and isolated apoA-I did not cross-react in the assay. Analytical recovery of apoB at different doses of very-low- and low-density lipoproteins were complete. Inter- and intra-assay coefficients of variation (CVs) averaged 7.4% and 6.0%, respectively. Blood from 20 subjects was collected into tubes containing EDTA alone or EDTA with antiproteolytic and antioxidant agents; one half of each plasma was separated immediately, half after 3 h at 4 degrees C. Regardless of the addition of protective agents or the time difference in separating plasma from other blood elements, freezing plasma at -70 degrees C decreased apoB content a similar amount, an average of 6.8%. This loss of apoB immunoreactivity was not related to apoB content in fresh plasma. Analysis of variance showed no differential effect on apoB content by the various additions to whole blood or plasma. No additional apoB content was lost in once-frozen aliquots of three human plasma pools during storage at -70 degrees C for up to 18 months. We conclude that concentrations of apoB in human plasma can be measured reliably after long-term storage, although the absolute value may decrease slightly as a result of freezing.

Alternate JournalClin Chem
PubMed ID2208707
Grant List55-N016 / / PHS HHS / United States