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Medium-term variability of the human serum metabolome in the Atherosclerosis Risk in Communities (ARIC) study.

TitleMedium-term variability of the human serum metabolome in the Atherosclerosis Risk in Communities (ARIC) study.
Publication TypeJournal Article
Year of Publication2014
AuthorsZheng Y, Yu B, Alexander D, Couper DJ
Secondary AuthorsBoerwinkle E
Date Published2014 Jun
KeywordsAtherosclerosis, Chromatography, Liquid, Female, Gas Chromatography-Mass Spectrometry, Humans, Male, Mass Spectrometry, Metabolome, Middle Aged, Serum

Metabolomics is a systems biology tool providing small molecule signatures of disease etiology. In order to estimate the biologic variability of the human serum metabolome, this study calculated intraclass correlation coefficients (ICCs) for 178 stably-detected metabolites measured by untargeted chromatography/mass spectrometry. We studied a subsample of 60 participants (57% males, 70% Caucasians, aged 73.77±5.3 years) in the Atherosclerosis Risk in Communities (ARIC) Study who provided two fasting serum samples 4-6 weeks apart. The median ICC across all metabolites was 0.60, and 82% of metabolites had at least fair variability (i.e., ICC>= 0.40). There was variation in the medium-term variability among metabolites, with those in the pathways of amino acid and lipid metabolism showing relatively high ICCs, and those in the carbohydrate pathway showing relatively low ICCs. The results of this study provide a valuable resource for future study design and outcome interpretation of mass spectrometry-based metabolomic studies in epidemiology.

Alternate JournalOMICS
PubMed ID24910946
PubMed Central IDPMC4048570
Grant ListHHSN268201100005C / / PHS HHS / United States
HHSN268201100009C / / PHS HHS / United States
3U01HG004402-02S1 / HG / NHGRI NIH HHS / United States
U01 HG004402 / HG / NHGRI NIH HHS / United States
HHSN268201100010C / / PHS HHS / United States
HHSN268201100008C / / PHS HHS / United States
HHSN268201100012C / / PHS HHS / United States
HHSN268201100007C / / PHS HHS / United States
HHSN268201100011C / / PHS HHS / United States
HHSN268201100006C / / PHS HHS / United States