|Title||An epigenome-wide study of obesity in African American youth and young adults: novel findings, replication in neutrophils, and relationship with gene expression.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Wang X, Pan Y, Zhu H, Hao G, Huang Y, Barnes V, Shi H, Snieder H, Pankow J, North K, Grove M, Guan W, Demerath EW, Dong Y|
|Secondary Authors||Su S|
|Keywords||Adolescent, Adult, African Americans, CpG Islands, DNA Methylation, Epigenomics, Female, Gene Expression Profiling, Gene Expression Regulation, Gene Regulatory Networks, Genome-Wide Association Study, Humans, Leukocytes, Male, Neutrophils, Obesity, Young Adult|
Background: We conducted an epigenome-wide association study (EWAS) on obesity in healthy youth and young adults and further examined to what extent identified signals influenced gene expression and were independent of cell type composition and obesity-related cardio-metabolic risk factors. Genome-wide DNA methylation data from leukocytes were obtained from 700 African Americans aged 14-36. We also measured genome-wide DNA methylation data from neutrophils as well as genome-wide gene expression data from leukocytes in a subset of samples ( = 188).
Results: The EWAS identified 76 obesity-related CpG sites in leukocytes with
Conclusion: In this study of youth and young adults, we identified 29 novel CpG sites associated with obesity and replicated the majority of the CpG sites previously identified. We further demonstrated that the majority of the obesity-related CpG sites in leukocytes were not driven by cell composition or obesity-related cardio-metabolic risk factors. We also provided the direct link between DNA methylation-gene expression-obesity for 5 genes.
|Alternate Journal||Clin Epigenetics|
|PubMed Central ID||PMC5756368|
|Grant List||R01 HL105689 / HL / NHLBI NIH HHS / United States |
R01 HL125577 / HL / NHLBI NIH HHS / United States