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Effect of Magnesium Supplementation on Circulating Biomarkers of Cardiovascular Disease.

TitleEffect of Magnesium Supplementation on Circulating Biomarkers of Cardiovascular Disease.
Publication TypeJournal Article
Year of Publication2020
AuthorsAlonso A, Chen LYee, Rudser KD, Norby FL, Rooney MR
Secondary AuthorsLutsey PL
JournalNutrients
Volume12
Issue6
Date Published2020 Jun 06
ISSN2072-6643
Abstract

(1) Background: Magnesium supplementation may be effective for the prevention of cardiometabolic diseases, but the mechanisms are unclear. Proteomic approaches can assist in identifying the underlying mechanisms. (2) Methods: We collected repeated blood samples from 52 individuals enrolled in a double-blind trial which randomized participants 1:1 to oral magnesium supplementation (400 mg magnesium/day in the form of magnesium oxide) or a matching placebo for 10 weeks. Plasma levels of 91 proteins were measured at baseline with follow-up samples using the Olink Cardiovascular Disease III proximity extension assay panel and were modeled as arbitrary units in a log scale. We evaluated the effect of oral magnesium supplementation for changes in protein levels and the baseline association between serum magnesium and protein levels. The Holm procedure was used to adjust for multiple comparisons. (3) Results: Participants were 73% women, 94% white, and had a mean age of 62. Changes in proteins did not significantly differ between the two intervention groups after correction for multiple comparisons. The most statistically significant effects were on myoglobin [difference -0.319 log units, 95% confidence interval (CI) (-0.550, -0.088), 0.008], tartrate-resistant acid phosphatase type 5 (-0.187, (-0.328, -0.045), 0.011), tumor necrosis factor ligand superfamily member 13B (-0.181, (-0.332, -0.031), 0.019), ST2 protein (-0.198, (-0.363, -0.032), 0.020), and interleukin-1 receptor type 1 (-0.144, (-0.273, -0.015), 0.029). Similarly, none of the associations of baseline serum magnesium with protein levels were significant after correction for multiple comparisons. (4) Conclusions: Although we did not identify statistically significant effects of oral magnesium supplementation in this relatively small study, this study demonstrates the value of proteomic approaches for the investigation of mechanisms underlying the beneficial effects of magnesium supplementation. Clinical Trials Registration: ClinicalTrials.gov NCT02837328.

DOI10.3390/nu12061697
Alternate JournalNutrients
PubMed ID32517192
PubMed Central IDPMC7352673
Grant ListT32HL007024 / HL / NHLBI NIH HHS / United States
T32 HL007779 / HL / NHLBI NIH HHS / United States
K24 HL148521 / HL / NHLBI NIH HHS / United States
T32HL007779 / HL / NHLBI NIH HHS / United States
16EIA26410001 / / American Heart Association /
UL1 TR002494 / TR / NCATS NIH HHS / United States
16EIA26410001 / AHA / American Heart Association-American Stroke Association / United States
K24HL148521 / HL / NHLBI NIH HHS / United States
T32 HL007024 / HL / NHLBI NIH HHS / United States
UL1TR002494 / TR / NCATS NIH HHS / United States