Title | Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study. |
Publication Type | Publication |
Year of Publication | 2020 |
Authors | Qadir S, Ellis O, Keizur EM, Stafylis C, Cortado R, Klausner JD |
Corporate Authors | Adolescent Trials Network(ATN) CARES Team |
Journal | J Microbiol Methods |
Volume | 168 |
Pagination | 105783 |
Date Published | 2020 Jan |
ISSN | 1872-8359 |
Keywords | DNA Gyrase, Drug Resistance, Bacterial, Feasibility Studies, Female, Genotype, Genotyping Techniques, Gonorrhea, Humans, Microbial Sensitivity Tests, Neisseria gonorrhoeae, Pharynx, Real-Time Polymerase Chain Reaction, Rectum, Serine-Type D-Ala-D-Ala Carboxypeptidase, Vagina |
Abstract | <p>Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche Cobas® and Hologic APTIMA™ clinical specimens. We studied whether similar methods could be used on remnant NG-positive Cepheid Xpert® specimens.</p> |
DOI | 10.1016/j.mimet.2019.105783 |
Alternate Journal | J Microbiol Methods |
PubMed ID | 31760050 |