Adolescents may accurately self-collect pharyngeal and rectal clinical specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae infection

TitleAdolescents may accurately self-collect pharyngeal and rectal clinical specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae infection
Publication TypePublication
Year of Publication2021
AuthorsVavala G, Goldbeck C, Bristow CC, Stafylis C, Adamson PC, Polanco D, Ocasio MA, Fournier J, Romero-Espinoza A, Flynn R, Bolan R, M Fern√°ndez I, Swendeman D, W Comulada S, Lee S-J, Rotheram-Borus MJane, Klausner JD
Corporate AuthorsAdolescent Medicine Trials Network(ATN) CARES Team
JournalPLoS One
Volume16
Issue9
Paginatione0255878
Date Published2021
ISSN1932-6203
KeywordsAdolescent, Child, Chlamydia Infections, Chlamydia trachomatis, Female, Gonorrhea, HIV Infections, Humans, Male, Neisseria gonorrhoeae, Pharynx, Polymerase Chain Reaction, Rectum, Self Care, Specimen Handling, Young Adult
Abstract

<p><b>BACKGROUND: </b>The COVID-19 pandemic illuminated the benefits of telemedicine. Self-collected specimens are a promising alternative to clinician-collected specimens when in-person testing is not feasible. In this study, we assessed the adequacy of self-collected pharyngeal and rectal specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae among individuals undergoing chlamydia and gonorrhea screening.</p><p><b>METHODS: </b>We used data from a large cohort study that included male and female adolescents between the ages of 12-24 years. We considered self-collected specimens adequate for clinical use if the human synthase gene (a control target of the assay) was detected in the specimen.</p><p><b>RESULTS: </b>In total, 2,458 specimens were included in the analysis. The human synthase gene was detected in 99.2% (2,439/2,458) of all self-collected specimens, 99.5% (1,108/1,114) of the pharyngeal specimens, and 99.0% (1,331/1,344) of the rectal specimens.</p><p><b>CONCLUSION: </b>Self-collected pharyngeal and rectal specimens demonstrated a very high proportion of human gene presence, suggesting that self-collection was accurate. A limitation of this study is that the sample adequacy control detects the presence or absence of the human hydroxymethylbilane synthase gene, but it does not indicate the specific anatomic origin of the human hydroxymethylbilane synthase gene. Self-collected specimens may be an appropriate alternative to clinician-collected specimens.</p>

DOI10.1371/journal.pone.0255878
Alternate JournalPLoS One
PubMed ID34570799
PubMed Central IDPMC8475974
Grant ListU19 HD089886 / HD / NICHD NIH HHS / United States