|Title||Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos.|
|Authors||Thyagarajan B, Howard AGreen, Durazo-Arvizu R, Eckfeldt JH, Gellman MD, Kim RS, Liu K, Mendez AJ, Penedo FJ, Talavera GA, Youngblood ME, Zhao L, Sotres-Alvarez D|
|Journal||Clin Chim Acta|
|Date Published||2016 Dec 01|
|Keywords||Adolescent, Adult, Biomarkers, Blood Cell Count, Blood Glucose, Fasting, Female, Ferritins, Health Surveys, Hispanic Americans, Humans, Male, Middle Aged, Sex Characteristics, triglycerides, Young Adult|
BACKGROUND: Biomarker variability, which includes within-individual variability (CV), between-individual variability (CV) and methodological variability (CV) is an important determinant of our ability to detect biomarker-disease associations. Estimates of CV and CV may be population specific and little data exists on biomarker variability in diverse Hispanic populations. Hence, we evaluated all 3 components of biomarker variability in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) using repeat blood collections (n=58) and duplicate blood measurements (n=761-929 depending on the biomarker).METHODS: We estimated the index of individuality (II) ((CV+CV)/CV) for 41 analytes and evaluated differences in the II across sexes and age groups.RESULTS: Biomarkers such as fasting glucose, triglycerides and ferritin had substantially higher inter-individual variability and lower II in HCHS/SOL as compared to the published literature. We also found significant sex-specific differences in the II for neutrophil count, platelet count, hemoglobin, % eosinophils and fasting glucose. The II for fasting insulin, post oral glucose tolerance test glucose and cystatin C was significantly higher among the 18-44y age group as compared to the 45+y age group.CONCLUSIONS: The implications of these findings for determining biomarker-disease associations in Hispanic populations need to be evaluated in future studies.
|Alternate Journal||Clin Chim Acta|
|PubMed Central ID||PMC5136507|
|Grant List||N01 HC065234 / HC / NHLBI NIH HHS / United States |
N01 HC065237 / HC / NHLBI NIH HHS / United States
P30 CA060553 / CA / NCI NIH HHS / United States
N01HC65233 / HL / NHLBI NIH HHS / United States
N01 HC065236 / HC / NHLBI NIH HHS / United States
P2C HD050924 / HD / NICHD NIH HHS / United States
N01 HC065235 / HC / NHLBI NIH HHS / United States
Analytical and biological variability in biomarker measurement in the Hispanic Community Health Study/Study of Latinos.